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KMID : 1189420170300020038
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2017 Volume.30 No. 2 p.38 ~ p.50
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Effects of Hot Water Extracts from Lonicera Japonica Flos Extracts on whitening using B16F10 cell lines
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Yu Pei-Yen
Kim Hye-Hwa
Lee Yu-Lim
Park Soo-Yeon
Jung Min-Young
Choi Jeong-Hwa
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Abstract
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Objectives : This study was designed to investigate effects of Lonicera Flos Extracts(LFE) on whitening using B16F10 cell lines.
Methods : In this experiment, we observed effects of LFE on cell viability, inhibitory effects of melanin synthesis, inhibitory effect on tyrosinase, tyrosinase activity, superoxide dismutase(SOD)-like activity and mRNA expression.
Results : In results, more than 2000㎍/㎖ of LFE treated group showed lowered cell viability rates significantly compared to albutin treated group. More than 2000㎍/㎖ of LFE treated groups were lower levels of melanin synthesis. Inhibitory effects of melanin production showed in 1000㎍/㎖ of LFE treated group. 1,000㎍/㎖ of LFE treated group significantly suppressed tyrosinase activities in vitro. LFE and albutin treated group significantly decreased tyrosinase activity compared to non treated group. SOD-like activity of LFE treated group was lower than vitamin C treated group but increased depending on concentration. 500㎍/㎖ of LFE treated group and 1,000 ㎍/㎖ of LFE treated group was significantly increased. Tyrosinase mRNA expression of α-MSH and LFE 250㎍/㎖ treated group significantly decreased compared to α-MSH treated group.
Conclusions : These results suggest that LFE can inhibit melanin synthesis through inhibitory action on tyrosinase
activity. And LFE suppressed tyrosinase activities B16F10 cells significantly. So I suggest LFE can apply to whitening.
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KEYWORD
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Whitening, Lonicerae Flos Extracts, LFE, B16F10 cells
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